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Starring John Abraham, Priyanka Chopra, Ravi Kishan and Sanjay Dutt,Â ÂKaram () is a 2005 Bollywood romantic action drama film written and directed by Sanjay Gupta, who also co-produced the film under the banner of his production house Premier International Films.Karam is a 2005 Bollywood romantic action drama film written and directed by Sanjay Gupta, who also co-produced the film under the banner of his production house Premier International Films.
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The study of mercury(II) and ammonium ions is of great importance in environmental and biological systems. A quadruple fluorescence lifetime imaging microscopic (FLIM) probe for the detection of Hg2+ and CH3NH3+ is described. Fluorophores including diphenylmethine green (DPMG), tetraphenylboron (TPB), and phenolic blue (PB) were chosen as the emission probes for Hg2+ and ammonium ion detection. In the probe design, the excess of Hg2+ and CH3NH3+ can bind with DPMG (or TPB or PB) via electron donor-acceptor (D-A) interactions, resulting in fluorescence quenching of the four probes via inner-filter effect. In addition, “turn-off” fluorescence of DPMG (or TPB or PB) was realized in the proximity of Hg2+ or CH3NH3+ with the assistance of metal-to-ligand charge transfer (MLCT) process. To facilitate the sequential detection of Hg2+ and ammonium ions in live cells, the excitation/emission intervals of DPMG and TPB were selected to be 580/645 and 480/590 nm, respectively, while the excitation/emission intervals of PB and DPMG were selected to be 320/645 and 480/590 nm, respectively. The responses of DPMG (or TPB) in Hg2+ and CH3NH3+ detection were monitored, respectively, using the probes with different excitation/emission intervals. The results exhibited that the DPMG probe (i.e., probe A)