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Winning Eleven 8 – Doxma mod V 3.0 (2008) · 17. – 04. – 09.Axon regeneration in long-term cultures of embryonic chick tectal and retinal explants depends on the initiation of neuronal cytoarchitecture.
Neurons taken from the optic tectum of chick embryos (stratum opticum) have been grown in vitro and allowed to develop to an advanced age in long-term cultures (LTC) (up to several years). After removal from the explants the cultured neurons have been examined by immunohistochemistry for neuronal markers and by electron microscopy for signs of axon regeneration. Specific antibodies against neurofilaments (NF) revealed that up to 15 days in vitro (DIV) NF-containing axons are present in vitro. On day 15 of LTC, numerous axons bearing NF accumulate at the margins of the explants and close to the culture dish bottom. Two weeks in vitro, i.e. beyond the period of time when the explants are transferred to dishes, NF-positive axons have regrown towards the explant periphery and in some cases regenerated directly through the collagen gel into the surrounding dish medium. The NF-positive axons establish a branching pattern and assume a retinotectal topography typical of the adult retina. The fine structure of these regenerating axons is identical to that of axons in adult tectum and retina. These observations support the hypothesis that in the absence of any supplementary growth factors or a specific organization of extracellular matrix surrounding the neuronal tissue explants from the optic tectum the axons can survive and regenerate in vitro when grafted onto foreign tissue.Garment finishing involves the fabrication of a garment, such as a shirt, using a variety of sewing techniques and garment fabrics, such as a woven fabric, an embroidered fabric or other suitable material, including knits. Garment finishing is used in the manufacture of garments for the civilian market as well as the military. Manufacturers of garments choose to finish the manufacture of their products in a certain way depending on the availability of materials and the cost of labor.
Hand sewing a suit jacket or dress shirt is becoming less popular. The popularity of quick-sew techniques, such as that disclosed in commonly-owned U.S. Pat. No. 7,922,813, often increases the amount of time a garment may take to manufacture. Manufacturers often prefer to finish clothing by
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